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Furthermore, a major spoligotype pattern of 17 (4.5%) strains characterized by lack of spacers 15 17 and 19 43 was not identified in SpolDB4.
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Two major spoligotype patterns (SB0120 and SB0131) were observed accounting for 36 isolates (69.2%), and 15 isolates (28.8%) cattle and lechwe respectively.
The ancestral East-African Indian family (EAI) is made up of at least five main subclades, whereas at least three major spoligotyping patterns are found within the Haarlem family (2).
Because only 1 major spoligotype and 2 similar MIRU patterns were found in this case series, spread of a predominant clone in Taiwan is likely.
The Beijing clade comprises at least 2 major subgroups, which share the characteristic spoligotype pattern (6 – 8 ): typical and atypical Beijing strains.
To determine the genotype similarity of strains sharing identical spoligotype patterns, we performed MIRU-VNTR 24 loci analysis on a subgroup of strains representing the major spoligotype clusters.
The only individual spoligotype pattern that was associated with HIV status was the most common Lineage 1 spoligotype, ST129, with 17/21 (81%) HIV positive.
In Ethiopia, a number of studies reported new strains with specific spoligotype pattern in cattle [32], [34] [36].
Each spoligotype pattern was classified into a binary code and the result was entered in a Microsoft Access database (Redmond, USA).
Each spoligotype pattern was classified into a binary code.
Beijing clade strains were defined by their spoligotype pattern (6 ).
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