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A comprehensive analysis of the distribution of all CSEPs showed that they are spread throughout the genome (Additional file 25), but two thirds of the 455 CSEPs located on 43 major sequence scaffolds were clustered family-wise (Additional file 25).
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Therefore, it is surprising that the chimeric tRNA performed just as well as the deleted tRNAArgCCU in terms of cell growth, despite the major sequence differences between the two tRNA scaffolds.
Preliminary results from stringent BLAT analysis of BES to sequence scaffolds can be viewed at SoyGD [ 4] Major challenges will have to be overcome in assigning function to the duplicated regions.
Finally, chromosomal scaffolds were produced by aligning contigs against the reference L. major sequence [ 13].
End-sequencing of the clones allow linking sequence scaffolds together.
The Tcas5.1 super scaffolds captured an additional 92 gaps between Tcas5.0 sequence scaffolds.
620 detected INDELs were located on unanchored sequence scaffolds.
Two ETS were identified on sequence scaffolds not yet assigned.
In addition, 99 SNPs were targeted to unanchored sequence scaffolds.
Additionally, by using SNPs from sequence scaffolds, other polymorphic markers within the same scaffolds could be selected for other populations.
The markers in the map derived from 1823 unique sequence scaffolds in the draft genome sequence.
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