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Finally, DNA barcode libraries are fully available as they are deposited in a major sequence database, and attached to a voucher specimen whose origin and current location are recorded [2], [3].
GenBank, a major sequence database and a component of the International Nucleotide Sequence Databases (INSD), doubles in size roughly every 18 months (2).
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It is apparent from Table 1 that the major sequence databases are not optimally suited to serve as barcoding engines as they presently stand; new techniques and strategies for data indexation and verification will have to be explored to address the above shortcomings [5], [22].
Unlike the major sequence databases, phylogenetic tree data does not have to be deposited in a database before it can be published.
The GO is the most widely used biomedical ontology and the utility of GO annotations is highlighted by the number of resources that provide them, including major sequence databases [such as UniProtKB (6)] and many of the prominent model organism databases (7).
The L. donovani genome has not yet been sequenced so that the present work had to be done with information of the L. major genome sequence database and occasional L. donovani protein sequences.
The Similarity Matrix of Proteins (SIMAP) database [ 118] provides a precalculated sequence similarity matrix for all proteins deposited at major public sequence databases.
To facilitate consistency in protein annotation, UniProt was launched in cooperation with three major protein sequence databases: Swiss-Prot, TrEMBL and PIR [ 24].
While the major public DNA sequence database groups at the NCBI and EMBL-EBI had developed nascent microarray data repositories, and work was under way to create a similar database at the DDBJ, submitting data to these databases was a considerable burden for authors.
One of the major causes for errors in protein identification is incompleteness of the peptide sequence database due to missed protein encoding genes and gene-models errors.
As a last control, the SNARE domain only of each identified L. major sequence was used for a BLASTP search of the L. major GeneDB database to try to identify any other possible candidate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com