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For this purpose, most of the publicly available major sequence assemblers – both for low-coverage long (Sanger) and high-coverage short (Illumina) reads technologies – are compared.
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Table 1 presents a nearly exhaustive list of the major state-of-the-art sequence assemblers organized by assembly paradigms.
As a consequence sequence assemblers typically produce multiple contigs.
Recently, next generation sequence assemblers were evaluated on bacterial datasets in the GAGE-B study.
A number of sequence assemblers have been developed recently to cope with sequences generated from the next generation sequencers.
Sequencing data were assembled, trimmed, and quality-checked by DNA Sequence Assembler v4 (2013), Heracle BioSoft, (www.DnaBaser.com).
The resulting sequences were then assembled by the Phrap program (Codon Code Aligner Sequence Assembler v3.0.1).
These data were assembled using the Celera Assembler (http://sourceforge.net/projects/wgs-assembler/) version 6.1 using the CABOG module, an overlap-layout-consensus sequence assembler that has been recently optimized for use with 454 Titanium paired-end sequence data.
The jump-start feature of the Celera Assembler was used for final sequence assembly achieving a final coverage of 21.2X.
Sequence assembly was performed first using the Newbler Assembler v1.1.02.15 (454 Life Science, Branford, CT).
The sequence assembly and scaffolding was done using the Newbler assembler software v2.9.
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