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The major secondary structure motifs (alpha-helixes and beta-strands) are closely aligned in the two structures.
For poliovirus IRES-mediated translation, the interaction of the cellular protein PCBP2 with a major secondary structure element (stem-loop IV) is required for gene expression.
Influence of the major secondary structure threshold on prediction.
Interestingly, the four complexes display all three major secondary structure types.
Thompson et al. (1994) showed that gaps occur far more often between major secondary structure elements than within.
For the time being, the bottom level of major secondary structure is kept at 75% for each considered work position, as described in the Material and Methods section.
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The method can be improved by using gap penalties specific to PB types with respect to major secondary structures.
In turn, the importance of the amide-I peak in monitoring secondary structures comes from the observation that hydrogen bonds formed in the backbone of proteins are implicated in the formation of the major secondary structures [77], [78].
Figure 1 a shows conserved regions, motifs, repeated units, and major secondary structures (both at DNA and RNA level).
As shown in the superimposed structures of the MedakaCea, FrogCea7, and LizardCeacam19 IgV-like domains (fig. 1), the major secondary structures are conserved.
Amplicons with major secondary structures were excluded, based on prediction made by the MFold program (http://mfold.rna.albany.edu/?q=mfold).
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