These experiments confirm that the MBL/MASP2K can generate functional thrombin, detected by activation of factor XIII and fibrinogen, which are two of the major protein substrates of thrombin.
SSeCKS is a major protein kinase C substrate with kinase scaffolding and metastasis-suppressor activity whose expression is severely downregulated in Src- and Ras-transformed fibroblast and epithelial cells and in human prostate, breast, and gastric cancers.
MARCKS is a major protein kinase C (PKC) substrate in a variety of cells including brain MP [77].
The subsequent identification of α-synuclein as the major protein component of the pathological substrate (Lewy bodies) placed α-synuclein at the center of PD research.
Likewise, caseins are the major protein sources in milk and cheese [ 1] and are thus prominent substrates for proteolysis by C. variabile, presumably carried out by secreted proteases.
Most of the intact enzymes were adsorbed to a high extent when the substrate concentration was raised, whereas most of the core enzymes, especially the major protein Cel7A, could be recovered in the supernatant.
In the current study, the amount, type, and activity of major allergen proteases secreted by Af was determined in response to either physiological substrates such as lung homogenate and mucin or a defined protein substrate such as casein.
The typical subdomain VIII includes a highly conserved PE motif and plays a major role in the recognition of peptide or protein substrates.
To clarify the inhibitory activities of 1 and 2 in physiological conditions, collagen, one of the major proteins in the human body, was used as a substrate instead of BSA.
The E3 ubiquitin ligases, including Nedd4 family proteins, are a major group of enzymes responsible for adding ubiquitin chains to protein substrates prior to their degradation.
