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The human Flt-1 gene contains two AUUAAA sequences just upstream of the major processing site of sFlt-1 mRNA.
We identified a major processing site (nucleotide position 112 in intron 13) of sFlt-1 mRNA produced in HMVECs.
In contrast, the soluble-form RNA, which was cleaved and polyadenylated at the major processing site (nucleotide position 112 of intron 13), was detected as a 651-bp band.
We first identified nucleotide position 112 of intron 13 as the major processing site of sFlt-1 mRNA in HMVECs by 3′-rapid amplification of cDNA ends (results not shown); the result was consistent with that of a previous study using HUVECs (human umbilical vein ECs) [ 7].
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To ensure processing at only the p2/NC site, we incorporated blocking mutations at all other major processing sites within pGPfs.
To this aim, we first analyzed in detail Kidins220/ARMS cleavage produced in vitro and in vivo, identifying a major calpain processing site in its C-terminal region (between amino acids 1669 and 1670) within a sequence motif highly conserved in vertebrates.
Yuleba is a small town and the site of a major processing facility for silica deposits.
These data indicate that β′-site cleavage is a major processing event that occurs with human APP in neuronal cultures.
We further analysed APP processing in neuronal cultures by short metabolic labelling experiments demonstrating that β′-site cleavage is a major processing event of wild-type (WT) human APP in neuronal cultures.
One major site is located at the helix 44 of the 20S, close to the processing site D, supporting previous results implicating Prp43 in the regulation of D site cleavage by endonuclease Nob1 [ 22].
Tri-Valley Growers, a major processing cooperative, went bankrupt in 2000.
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