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Embryos injected with mt2 MO or mt2 spbMO, showed brain necrosis but no other major morphological defects (Fig. 1).
40 Altogether, our results show that major morphological defects are present in NMJs of patients with ALS even at the early stages.
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Nevertheless, the upregulation of the tip cell specification program is unable to compensate for the sprouting defect in Nrp1 null mutants, arguing for a major morphological defect that prevents proper execution of tip cell behavior.
The post-thaw semen quality of the five bulls from group 1 and eight bulls of group 2 are summarised in Tables 1 and 2. No statistical differences were seen between the groups for sperm concentration and the percentage of minor morphological defects; nevertheless, as expected, total major defects and the percentage of PCDs were higher for group 2 - PCD (P < 0.05) (Table 1).
GFP-nestin-positive radial glial fibers, containing the R1 construct, did not show any major morphological differences or glial endfeet defects compared with GFP-nestin-positive radial glia expressing a control construct (Fig. 3A).
The results showed good crystalline behaviors and morphologies without any identifiable morphological defects.
By using optical mapping and submaximal levels of hadp1 knockdown, we observed profound effects on Ca2+ handling and on action potential duration in the absence of morphological defects, suggesting that Hadp1 plays a major role in the regulation of intracellular Ca2+ handling in the heart.
Whatever the reasons, the study shows that major morphological changes can occur in a species right under our noses.
These mice were viable, with no apparent morphological defects.
It is also associated with major morphological and physiological changes.
The orbit is a second major morphological landmark.
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Justyna Jupowicz-Kozak
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