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The genetic variation found between the major molecular types of C. neoformans var.
Clinical and environmental isolates clustered together in the respective major molecular types as previously determined in an analysis of six genetic loci (ITS1/2, IGS1, CNLAC1, RPB1, RPB2 and TEF1) [14].
The herein presented multigene sequencing data revealed seven major haploid lineages within the Cryptococcus species complex and provide further evidence to consider these major molecular types as individual varieties, if not species.
The ILD/PHT P value for each gene combined individually (P values ranged from 0.010 for ACT∶PLB1 to 0.835 for URA5∶IDE) and all genes combined (P = 0.002) showed no significant incongruence using a cut off point of 0.0001 [30], indicating a low degree or absence of recombination between the major molecular types, when only strains representative of the main lineages were used.
neoformans into four major molecular types.
The results of this study permitted us to determine the major molecular types and their distribution within each participating country.
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VGI is the major molecular type recovered from clinical, veterinary and environmental samples from eastern Australia where human populations are most concentrated [24].
The CTX-M ESBL was the dominant enzyme, and CTX-M-14 was the major molecular type detected in community-onset infections.
Initially, individual PCR fingerprints were visually compared to those of the standard strains, amplified in parallel, to determine the major molecular type for each isolate.
The latter studies generally made use of an F1 population as the plant material, with amplified fragment length polymorphisms (AFLP), simple sequence repeats (SSR), and single nucleotide polymorphisms (SNP) being the three major molecular marker types for map construction [ 6- 16].
Genotyping methods have identified major haploid molecular types of C. neoformans (VNI, VNII, VNB and VNIV) and of C. gattii (VGI, VGII, VGIII and VGIV).
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