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To mimic physiological environment and to simulate "injury", cells in a well were maintained not only on tissue culture plastic but also on collagens I and IV, major matrix components in musculoskeletal and adipose tissues respectively, and subjected to a severe thermal stress.
Pectins are the major matrix components of dicotyledonous cell walls.
In addition, these alterations were accompanied by a reduction of immunohistochemical staining intensity in other major matrix components, such as hyaluronan, neurocan, brevican and phosphacan.
To determine the effect of GSK3 inhibition on ECM deposition, we measured expression levels of collagen-1 and fibronectin, major matrix components.
It should be noted that the amounts of major matrix components (i.e., collagen-I, collagen-II and GAGs) in AFSCs cultured on the aligned and random scaffolds were comparable to those in native AF tissue in rabbits.
Applying this form of microscopy to the analysis of both the protein and polysaccharide components of the matrix (for example using fluorescently labelled amino acids or sugars) may reveal further detail as to how these two major matrix components interact and provide the incredible structural integrity to the biofilm.
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The morphology and mechanical properties of ternary blends where PC is a major matrix component rather than simply a dispersing agent are also examined.
It has been shown that laminin-2 was a major matrix component of the PNS which could induce cell spreading and/or myelination [29].
In cells cultured on aligned scaffolds, the gene expression and protein production of collagen-I, the major matrix component of AF tissue, was markedly higher than that of cells grown on random scaffolds.
The biofilm-forming capacity varied among strains, and the media composition drastically altered biofilm biomasses and major biofilm matrix components.
Micropatterned cellular co-cultures were fabricated using three major extracellular matrix components: hyaluronic acid (HA), fibronectin (FN) and collagen.
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