Particularly when using laser intensities beyond the fluorescence saturation limit, phototoxic reactions introduce major limitations in live cell fluorescence microscopy [3].
Furthermore, we emphasized the issue of two major limitations in the discussion section: the limited number of subjects as well as the lack of an active control group.
Unfortunately, the high cost of crystal detectors and limited axial field of view of current systems pose major limitations in this clinical application.
However, one of the major limitations in translation of stem cell technologies for clinical applications is limited cell survival and growth upon implantation.
One of the major limitations in the genetic manipulation of X. dendrorhous is the limited number of molecular tools available to transform and engineer this yeast.
Abiotic stress conditions are the major limitations in modern agriculture.
Chatter is one of the major limitations in milling operations causing poor quality and reduced productivity.
The two major limitations in [IEEE Trans Autom Control 1996; 41(11): 1691–1693] are eliminated.
Major limitations in the study design, including the small size of study groups, affected the reliability of these studies.
Despite recent progress with drug-eluting stents, restenosis and thrombosis after endovascular intervention are still major limitations in the treatment of cardiovascular diseases.
The condition is surrounded by taboo; it can have a devastating impact on quality of life and lead to major limitations in daily life.
