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This study, however, had one major limitation which must be noted.
Despite many advantages of graphene-based electrodes, there remains a major limitation, which is low value of specific capacitance.
First of all, recall bias was the major limitation, which could be expected in population-based retrospective studies.
The approach is denoted expanded PLS (E-PLS) and takes advantage of the simplicity of use of two-way models for batch processes and tackles its major limitation which is over-fitting.
The major limitation, which arised from the retrospective nature of the study, was that data of normal colorectal mucosa were not included in the statistical models.
A major limitation, which applies to almost all observational studies evaluating PCT, is that there is no accurate gold standard for the outcome definition of bacterial infection.
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However, the study suffered from assumptions made due to lack of information on the age at onset, relapse frequency or type of symptoms at onset of disease presented major limitations which the authors argued could affect the favorability of cost-effectiveness ratios.
* those with major physical limitations which (a) limited their ability to engage in physical activity a lot and (b) estimated to last for more than 4 weeks or unsure.
The reliance on volunteers is a major limitation in this study, which may limit the generalization of the study findings.
Normal tissues injury or damage is the major limitation of using CP, which gives rise to numerous side effects; CP treatment also results in the production of reactive oxygen species (ROS), which cause peroxidative damage to vital organs [ 21].
Although it has been a proven technology for removal of contaminants including heavy metals, it has a major limitation to its application, which is the cost of the adsorbent.
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