The major isoform present in adult human epidermis is ΔNp63 α, expressed at high levels in the basal layer and at lower levels in suprabasal keratinocytes.
Since MARK4 was the major isoform present in GVDs and most strongly elevated in AD cases, we chose to continue to study the MARK4 isoform in relation to neurofibrillary changes.
α-Smooth muscle actin specifically labels the α-smooth muscle isoform of actin and does not react with other major actin isoforms present in fibroblasts or epithelial cells, striated muscle, myocardium, or gamma-smooth muscle isoform.
However, there is only a single SUMO protein in yeast, whereas there are three major protein conjugating isoforms present in mammals; these are SUMO-1, and the highly similar SUMO-2 and SUMO-3, which are often refer to as SUMO-2/3.
The phenotype observed in cystinotic cells is specific for LAMP2A since the other major LAMP2 isoform present in fibroblasts, LAMP2B, is normally expressed and localized at lysosomes in Ctns − / − cells.
The major isoforms of HLA-G present in serum are soluble HLA-G1 and HLA-G5 generated either by shedding or proteolytic cleavage of the membrane bound isoform or by secretion of a soluble isoform[6].
To address the expression profile of the two major isoforms of p63, in the present study, we have utilized ΔN and TA specific antibodies.
They are also present in two major isoforms in low basal levels in various organs of adults in several species.
The nuclear localization of the long isoforms requires also a second domain termed Nuclear Domain A (NDA), which is an internal amino acid stretch of the common exon V also present in the major isoforms [ 10].
Table 2 is a quantitation of each glycan structure present on the five major isoforms for each patient group.
