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The lymphocyte tyrosine kinase Lck is the major interaction partner of Tip and phosphorylates Tip at specific tyrosine residues.
It is conceivable that the regulation of VPS34 stability is independent of Beclin 1, whereas the stability of Beclin 1 itself depends on its major interaction partner VPS34.
One major interaction partner of the mammalian AP-2 complex is Eps15, which was first discovered as a substrate of the epidermal growth factor receptor tyrosine kinase activity (Benmerah et al. 1995; Fazioli et al. 1993), but later also shown to interact directly with phocein and NDPK (Baillat et al. 2002).
These results demonstrate that the ATPase activity is critical for determining PICH localization, and they confirm that PICH is a major interaction partner of Plk1 on mitotic chromosomes (Baumann et al. 2007; Hutchins et al. 2010; Leng et al. 2008; Santamaria et al. 2007).
Similar(56)
In summary, the split YFP and Y2H results indicate that NH1 and NH3 are the major interaction partners of rice TGA proteins (depicted as solid dark lines).
Ferguson-Miller et al. have, indeed, identified residues Glu148, Glu157, Asp195, and Asp214 (all in subunit II of CcO from R. sphaeroides) as the major interaction partners of Cc [ 37].
Mass spectrometry of the isolated, high molecular, cross-linked stomatin complexes revealed the major interaction partners as glucose transporter-1 (GLUT1), anion exchanger (band 3), and water channel (aquaporin-1).
Using YFPC-fused RH proteins and YFPN fusions of the TGA proteins determined to be the major interaction partners of NH proteins from the split YFP assay (rTGA2.1, rTGA2.2, rTGA2.3, rLG2, TGAL2, and TGAL4), we tested whether the introduction of various NH proteins (non-YFP fusions) was able to facilitate interaction between YFPN and YFPC fusions, yielding a fluorescence signal.
SEP3 strongly activates the expression of the A gene AP1, B gene AP3, and C gene AG; these factors are the major protein interaction partners of SEP3, suggesting that SEP3 can activate the flower developmental program by enhancing the expression of its interaction partners [ 18].
These two terminal peptides wrap around the partner proteins forming the major interaction between Ran and RanBD1.
Another interaction partner was pigment epithelium-derived factor (PEDF), which is the major circulating inhibitor of plasmin.
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