Functional annotation clustering analysis of the early down-regulated genes in both wounds identified two major overlapping functional terms: DNA dependent transcription and DNA binding (Table 5).
However, the major functional groups (GO-term) regulated are neuronal, such as "synaptic transmission", "neurotransmitter secretion", "axon guidance" or "nervous system development".
We further classified these genes into 11 major functional categories following the GO terms in 'biological process' with all remaining genes grouped into 'other functions' or 'unknown'unknown
In addition, we used Blast2GO to determine the functional annotation based on GO terms into the three major functional categories: Cellular Component (CC), Biological Process (BP) and Molecular Function (MF).
The unigenes were classified into three major functional categories: biological process, molecular function, and cellular component, according to the standard Gene Ontology terms (GO; ).
The major functional RNAse in the mitochondria mechanism is termed the mitochondrial degradasome or mtEXO and consists of a 1 1 ratio of Dss1, a member of the RNaseII family, and Suv3, a DEVH family member.
Taking into account GO term and intra-connectivity, the major functional modules contain an enrichment of cell cycle, extracellular matrix, glycolysis/fast twitch muscle subunits, slow twitch muscle subunits, nuclear-encoded mitochondrial and mitochondrially-encoded mitochondrial genes.
Long-term disease can lead to major functional disability; therefore, early diagnosis and determination of risk factors are important in effective disease management.
The lack of major functional benefit can be rationalized by the paucity of long-term engrafted GFP+ cardiomyocytes.
