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More to the point, a sub-network analysis was done to determine major functional clusters in human DRIN that govern key neurological pathways.
Notably, we found two major functional clusters in the pathway crosstalk network.
GO analysis revealed chemotaxis, cell migration and cytoskeleton organization as the major functional clusters affected within the upregulated genes.
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Other major functional groups in this cluster included apoptosis (4 genes), cell surface receptors (7 genes) and intercellular signalling proteins (6 genes).
Tardigrade genomes invest in stress-specific adaptations, this includes major sequence related protein clusters, functional clusters for stress as well as specific regulatory elements in mRNA.
Among 307 up-regulated genes, 221 (72.0%) could be classified into 4 major functional groups according to the Cluster of Orthologous Groups of proteins (COGs) database.
Differentially expressed transcripts clustered in five major functional categories: (1) regulation of cell proliferation; (2) regulation of programmed cell death; (3) response to oxidative stress; (4) cell adhesion, and (5) chemical homeostasis.
Phylogenetic analysis revealed that the candidate CstyEsts clustered within all three of the major functional groups of the esterase gene family (based on the classification system of [ 47]).
If a particular amino acid site is variable in both clusters, it is unlikely to have any major functional role in either paralog.
Then, cluster analysis was utilized to detect the major functional modules related to nicotine addiction in the PPI network.
The Clusters of Orthologous Groups (COG) database [ 62] defines four major functional categories: "information storage and processing", "cellular processes and signaling", "metabolism", and "poorly characterized", which are further subdivided into 25 functional categories.
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