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Semi-quantitative RT-PCR was used to prepare expression ratios Sor60/3153A from Ch5a with three genes representing three major expression levels on Ch5b.
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Overall, this suggests that the majority of expression changes within aneuploids are of relatively small magnitude and that perturbation of trans-acting regulatory networks rarely results in major expression level changes.
Interestingly, major satellite expression level is much lower in male than female PGCs in Tet1-KO, although 5hmC is undetectable in both sexes.
Therefore, the residual changes in gene-expression are attributable to our three major variables, 'infection', 'time' and 'temperature'; all of which contribute to major changes in expression levels in the S. tropicalis spleen.
The mammalian genes are also expressed in domains both dorsoventrally and rostrocaudally restricted, with major changes in expression levels coinciding with compartment boundaries [42].
We have established that most Wolbachia genes are expressed in all D. melanogaster life cycle stages, but that major changes in expression levels of Wolbachia genes are rare when studied simultaneously across all D. melanogaster tissues.
Gender and age were the two major factors influencing expression levels (807 gene expressions were affected by gender and 396 by age).
Copy number can be a major determinant of expression levels for some genes.
In a postmortem specimen study of major depression, decreased expression levels of subunits of mitochondrial complex I were found in the cerebellum.
For 21 genes, no major changes in expression levels were observed — neither if expression levels in postischemic retinae were compared with the respective control eyes, nor if expression levels were compared between Wt and P2Y1R-KO retinae.
No major differences in expression levels of these activation markers could be detected between CD4+CD25+ T cells from IFN-γR KO mice and those from wild-type mice, whether naive or immunised.
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