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Then I have bullet points for major comments and for minor comments.
We addressed this point in point 3 of the major comments and added the results to Author response image 1.
Major comments and criticism: Original (raw) data and processed data used for model construction and analyses (e.g., matrix of joint publication) are not available.
The major and minor comments are listed below and all questions seem reasonable, and the experiments suggested by referee 1 in major comments and referee 2 in major (points 1 and 2) and minor comments can be readily done.
The reviewers have discussed their major comments and arrived at the following consensus comments that will need to be addressed in a revised submission. 1) More analysis is needed to define the phenotypes of Nhe2 overexpression and loss of function.
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Reviewer 2: I only had one major comment and this is with how the paper is framed.
I have one major comment and several minor issues: Major: the manuscript does not, but should, deal to any extent with possible side-effects of creatine supplementation.
Major comments: The number and organization of the figures and movies interferes with the readability of the manuscript.
Reviewer #1 (General assessment and major comments): This manuscript from Hadley and co-workers describes an exciting new technique called STOMP, which promises to revolutionise targeted microproteomics.
Reviewer #1 summary and major comments: The study by Herman and collaborators aims at characterizing the fidelity of optogenetically-induced spike in inhibitory and principal neurons from the mouse brain using in vitro and in vivo electrophysiology.
AC and JYR wrote the manuscript, with major comments from FK, PEH and CK.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com