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The new teosinte Z. nicaraguensis was classified into the first major cluster and thus considered to be a section Luxuriantes species.
The three cell lines with the greatest (>50%) reduction in anchorage-independent colony formation, SKRC45, CAKI-1 and 769-P were in the second major cluster and apparently did not demonstrate a strong hypermethylation phenotype.
Matched samples were in the same terminal cluster for patients 1 and 4, with the samples from patient 1 grouped in the benign major cluster and those from patient 4 in the cancer major cluster.
To confirm the validity of miRNA array data, we had previously measured miRNA expression levels by real-time RT-PCR in our laboratory available from 20 miRNAs differentially expressed between the major cluster and the minor cluster for four ER-positive cell lines and four ER-negative cell lines.
At this level of protein sequence identity, three major subgroups and a number of minor subgroups could be identified as illustrated in Figure 2 and four representative proteins, one from each major cluster and one from a single minor cluster, were selected for functional characterization.
Sequence logos of the major cluster and one secondary cluster (the closest one according to row correlation) for each of four groups are shown in Figure 4. Sequence logos of clusters 2, 4 and 21 show strong sequence similarity to regions of actual targets of examined antibodies (listed in Table 1).
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The analysis using t-distributed stochastic neighbor embedding (t-SNE) was performed to visualize the major clusters and the gene expression distribution (Supplementary Figure 10).
The analysis indicated that twelve strains were organized into two major clusters, and that all strains of each subspecies were confined to one cluster.
There are two major clusters and several distinct locations such as Station 239 and stations nearby Station 220 are surfaced in this mode throughout the week.
The D1 regions of the 105 coa were classified into 12 major clusters and those belonging to each cluster showed nucleotide identities with more than 90% (figure 2).
Breeding germplasm was separated into four major clusters and landraces.
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