Patient 2 was diagnosed with LGL leukemia at the age of 76 years and harbored a major T-cell clone that constituted 73% of all CD8+ cells.
In comparison with the resolutions of PFGE patterns described by Huang [ 7], genotype A, C, and D, the major clones in that report, consisted of 81.3% of the isolates in the present study (Table 3).
Cloning and sequencing of this major band revealed a 2274 bp clone that represented a truncated copy of SmTRC1, which we designated SmTRC1d1.
In S. aureus isolates, major pandemic MRSA clones that are responsible for most hospital-acquired infections are found in multiple genetically unrelated lineages, though most previously identified pandemic clones are found in clonal complex 8 (38, 39).
Although the pediatric clone was a major clone in 1992 1997 in that particular hospital, it was detected in a single isolate only in the national study performed in 2006 [25].
Among MRSA strains, results indicated that a major clone matching the Lyon clone (66%) was widely distributed.
The University of Edinburgh study looked at the genetic make-up of more than 80 variants of a major clone of MRSA found in hospitals that were collected from patients during a 53-year period.
23 However, more recent evidence casts doubt on PVL being a major virulence determinant, as clones that lack PVL genes still show significant virulence.
We reason that if a subclone replaces the major clone during tumor evolution, it is because of its higher fitness.
Genotyping analysis of clinical isolates by PFGE showed that 104 isolates belonged to a single major clone (clone B), 29 belonged to a second clone (clone A), and 2 belonged to 2 clones (clones C and D).
