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Identical tree topologies were obtained for all major clades using each method, and adjusting the search parameters for each.
Douzery et al. (2004) measured the evolutionary time of the major clades using a large dataset of 129 proteins from 36 eukaryotes.
The most recent phylogenetic analysis of the group recovered four major clades using a combined analysis of four nuclear (ITS, three CYCLOIDEA homologues) and two chloroplast (trnH-psbA and matK) regions (Ojeda et al. 2012).
In Viburnum it is generally possible to confidently distinguish species belonging to the different major clades using the core barcodes, but the failure rate is very high when we consider close relatives within these clades.
The ML tree of msp1 shows two major clades using P. gonderi as an outgroup, one for P. vivax and the other for the seven Asian monkey parasite species.
From an analysis of 1969 bp COI and COII sequences from 55 Agrodiaetus species, Kandul et al. [ 19] proposed a classification of 12 major clades using Maximum Parsimony and Bayesian inference most of which have high bootstrap and Bayesian support.
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The BAHD family has been previously organized into five major phylogenetic clades, using 46 biochemically or genetically characterized members [ 2].
Molecular phylogenetic dating of the ages of the major grass clades, using the fossil pollen to set a minimum age for the diversification of the crown group of grasses, suggests that the BEP and PACCAD clades had themselves diversified by approx. 35 40 mya (but with wide confidence intervals) (Bremer, 2002).
Here we comprehensively assess the potential risk to mammals of a diverse panel of A/H9N2 viruses, representing the major H9N2 clades, using a combination of in vitro assays (e.g., antiviral susceptibility and virus growth in primary differentiated human airway cells) and in vivo assays (e.g., replication, transmission and/or pathogenicity of viruses in ducks, pigs, mice and ferrets).
When considering the major clades obtained using the maximum likelihood and the distance phylogenetic methods (Additional file 12), the trees architectures did not changed significantly.
After applying the BIC and AIC test and constructing a phylogenetic tree using the ML procedure to classify the genes into five major clades, we used the JTT model of amino acid substitutions to evaluate phylogeny (Fig. 1).
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