RUE was derived from the ratio of the optical density of one kind of carbon substrate with the total optical density of all other carbon substrates among the seven major carbon substrates including monosaccharides and glycosides, amino acids, alcohols, amines, polymerization saccharides, lipids, and organic acids (Wei et al. 2008).
Arthrobacter strains are well-equipped with enzymes required for the catabolism of major carbon substrates present at the cheese surface.
Like the environmental Arthrobacter strains, A. arilaitensis Re117 is well-equipped with enzymes required for the catabolism of major carbon substrates present at cheese surfaces such as fatty acids, amino acids and lactic acid.
Only the central metabolic pathways (glycolysis, glutaminolysis and amino acid pathways) were analyzed taking into account consumption and production of major carbon substrates and by-products.
In the batch culture, the two major carbon substrates, glucose and glutamine, were not limiting nutrients since their concentrations at the end of the culture were of 7 mM and 0.54 mM respectively (corresponding to 21%andd 13.5% of their initial concentrations).
Starch was the major carbon source in the substrate, simulating wastewater containing carbohydrate.
While starchy substrates or molasses are major carbon feedstocks for fermentative production of 1-butanol in clostridia or heterologous microorganisms, interest is growing in employing novel substrates that are economically competitive with petrochemical synthesis yet at the same time non-competitive with food for human consumption [ 12].
Interestingly, no major difference was observed when cellobiose was the sole carbon substrate: specific growth rates measured with both substrate were almost similar, and no significant change in fermentation patterns (acidogenic phase) was observed.
Major components of PHAs produced by P.putida LS46 are either 3-hydroxyoctonoate or 3-hydroxydecanoate depending on the carbon substrate used for PHAs production (Sharma et al. [2011]).
The primary carbon substrate is CH4.
