Peptide arrays further identified a major binding determinant for PKAc was located in exons 5 and 6 of AKIP 1A (Fig. 5A).
The amino acid sequence of VH3-CDR3, thypervariableble region which in most cases acts as a major binding determinant [6] [8], is a unique and distinctive feature of each member of the repository.
The AFF4 2–363 and 33 67 fragments bound to CycT1 with similar affinities (Table 2), suggesting that the ordered AFF4 segment in the crystal structure captures the major CycT1 binding determinants.
The major IGF-binding determinants are located in the N- and C- domains and they interact with residues on the IGFs that are also important for IGF-1R binding (and IR-A binding in the case of IGF-II).
major binding saccharides.
VHL has two well-defined TRiC binding determinants.
However, their IgE-binding determinants remain unknown.
In contrast, the ability to adopt the shifted conformation of stem-loop I is a major determinant of binding: mutants that cannot adopt this conformation bind much more weakly than wild-type and mutants with a constitutively shifted stem-loop I bind much more strongly.
These diamino moieties, and the requisite hydrogen bonds formed between them, or between the observed water molecules, with conserved protein atoms, are known to be major determinants of binding in the substrate pocket.
This residue is a major determinant of substrate binding specificity toward a productive binding mode.
