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Exact(6)

The bands for PLs and FFAs were eluted with methanol: chloroform (1 1, v/v), and the upper two major bands corresponding to TAGs and SE respectively, were eluted with chloroform.

Two major bands corresponding to ≈26 kDa and ≈29 kDa proteins could be seen (Fig. 1D, lane 2) in addition to minor non-specific tryptic digestion products.

Major bands corresponding to the expected apparent molecular masses of the respective fusion proteins were observed, with fainter high MW bands likely reflecting oligomerized Zera fusions.

As shown in Figure 7B, TS-jPCR resulted in the amplification of single major bands corresponding to the expected sizes of IgG- and IgK-expression constructs.

We were able to distinguish two major bands, corresponding to the isoforms of the β-glucosidases previously described [ 17], and three minor bands (estimated purity yield close to 85% of the total protein content; data not shown).

Understandably, if a single M. agalactiae strain, similar to PG2 reference strain, was circulating in the P.A., it seems reasonable to suspect that all serum samples from infected animals would express the same IBT profile with four major bands corresponding to stable proteins and a 27 kDa band, as confirmed by analysis of the 22 sera from infected reference animals.

Similar(54)

A two-step batch purification combining ion exchange and immunoaffinity chromatography, followed by Western blot analysis, revealed a complex pattern with a major band corresponding to the standard form of CD44 and minor bands that may correspond to larger variant forms.

After induction with methanol we detected significant levels of unprocessed full-length MBP-fur-GrB in supernatants of pPIC9-MBP-fur-GrB clones in addition to the major band corresponding to processed GrB (Fig. 5B).

As seen in Fig. 9A, the mutant A302G (lane 5) completely abrogated the aggregation propensity of FadD13, as analyzed by native polyacrylamide electrophoresis, resulting in a single major band corresponding to dimeric species as opposed to the presence of aggregates in addition to the predominance of dimeric species in the case of native FadD13 (lane 1).

For FNR the MLS seems to be processed correctly as shown by WB analysis were we found a major band corresponding to the size of the mature protein and with only a small proportion remaining as the full-length, non-processed form.

To characterize this major band corresponding to the partially edited A6 mRNA, it was cut from the gel, cloned, and sequenced from the lanes of both KREPA3-RKO and RKO-A3ZFm2-myc cells with KREPA3 Reg expressed (E) or repressed (R) for 3 days.

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