The Western blot analysis also demonstrated that there are no isolated major bacterial antigens against which the host mounts humoral response.
A few studies conducted in different parts of the country have shown that Campylobacter, Salmonella, Shigella and rotavirus are some of the major bacterial and viral pathogens isolated from stool of diarrheic patients, most of them children under 5 years of age [ 17– 20].
According to PCR amplification, sequencing of 16S rRNA gene and its alignment in BLAST revealed that the isolates belonged to three major bacterial classes: Bacilli, gamma-proteobacteria and alpha-proteobacteria. Isolates belonged to six genera; Acinetobacter, Bacillus, Pseudomonas, Bosea, Azospirillum and Nitratireductar.
Culture-independent bacterial identification Genomic DNA isolation from bacterial isolates.
Genomic DNA isolation from bacterial isolates.
MLST schemes have been developed for all major human pathogens, and it has been the standard technique for classification of bacterial isolates [ 30, 31].
In addition to two major bacterial pathogens, S. pneumoniae (n = 4) and H. influenzae (n = 1), the most commonly isolated bacteria was B. cepacia group (n = 9).
The range of G+C contents of plasmids isolated corresponds with the overall range in genomic G+C content observed among the major bacterial divisions comprising the human gut microbiota.
Pasteurella multocida is believed as a major bacterial agent that complicates swine influenza virus infections, and still remains the most common respiratory bacterium isolated in cases of PRDC [ 9, 13].
Screening of bacterial isolates for proteolytic activity.
