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In the present paper, two major analyses are achieved.
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The major analyses were based on the information derived from the abstract evaluation and the data base searches.
32 All the relevant variables included in the major analyses were used in the multiple imputation models, as was the outcome (age at death).
In order to elucidate the influence of HPV infection, major analyses were repeated: (1) among all cases and controls, (2) among HPV-positive vs HPV-negative control women, and (3) among HPV-positive cases vs HPV-positive control women only.
Since oral-pharyngeal and oesophageal cancers are more frequent in men, and to avoid heterogeneity between sexes of the covariates included in the model, the major analyses were restricted to men and the results on female subjects were given in the text only.
All major analyses have been completed and were presented at a conference in May 2012 to an audience of clinicians, regulators, scientists, and representatives from the private sector.
Major analyses have been executed in order to optimize the design.
Major ion analyses were done at the Isotope Hydrology Laboratory of Kumamoto University, Japan, for major ions, while dissolved silica (SiO2) was analyzed at the National Agriculture Center for Kyushu-Okinawa Region, Kumamoto.
Two major contrast analyses were performed.
Primary and major secondary analyses were intent-to-treat.
Hypothesis testing in the primary and major secondary analyses was controlled by sequential testing to adjust for multiplicity.
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