A final group of allergen orthologues, including the major allergens Asp f 1 and Alt a 1, appear to be present in only a limited number of species.
In the present study, the lines with reduced levels of the major allergens and Major x HMW lines showed strong GbN-1 characteristics because rice plants were grown in small pots, in which rice could not set their roots deep enough.
This might be due to the relatively low number of related proteins present in mature seeds compared to some of the major allergens, such as gliadins.
A. Western blot of the major allergens (26 kDa, 33 kDa, and 14 16 kDa) and the HMW allergens (52 kDa and 63 kDa allergens).
We crossed the rice with reduced HMW allergens and with reduced major allergens, and all major and HMW allergens were substantially reduced in the progeny of the crossed rice.
We suppressed the levels of these HMW allergens by RNAi and crossed the RNAi rice with the transgenic rice with reduced levels of the major allergens.
We crossed transgenic rice lines with reduced levels of the HMW allergens in endosperm and embryo, and transgenic lines with reduced levels of the major allergens.
NT, non-transgenic Koshihikari; Major, the rice lines with reduced levels of major allergens; HMW, the rice lines with reduced levels of the HMW allergens (endosperm- and embryo-specific suppression); Major x HMW, the rice lines with reduced levels of the major and the HMW allergens.
NT, non-transgenic Koshihikari; Major, the rice lines with reduced levels of the major allergens; HMW, the rice lines with reduced levels of the HMW allergens (endosperm and embryo-specific suppression); Major x HMW, the rice lines with reduced levels of the major and the HMW allergens.
We successfully generated transgenic rice with reduced content of the major and the HMW allergens, which showed reduced IgE binding to sera of a portion of rice allergy patients who are allergic to proteins other than the major allergens.
