Sentence examples for major allele differences from inspiring English sources

The exact positions of these indel markers in the maize genome, as well as the primer sequences, amplicon lengths, PIC values, major allele differences, number of alleles, and number of e-PCR products in the maize genomes evaluated are presented in Additional files: 3 and 4.

Thus, the genome-wide and easily used InDel markers with polymorphism information content (PIC) ≥ 0.5 and major allele differences (size difference between the most and second most frequent alleles) ≥ 3 bp or 8 bp, which were clustered by the major groups of O. sativa (Japonica and Indica), O. rufipogon and O. nivara, will have significant practical implications in rice breeding.

Of these highly polymorphic InDels, we also selected InDels with major allele differences (the size difference between the most and second most frequent alleles) ≥ 3 bp or 8 bp for primer design, which provided a more flexible choice for researchers.

The indel markers had PIC values ≥ 0.5, and major allele differences ≥ 3 bp.

One hundred pairs of primers (with PIC values ≥ 0.45 and major allele differences ≥ 3 bp) were selected, and DNA from twenty new maize inbred lines was used as the template for indel polymorphism validation.

Primer pairs were also designed for 29,403 unique indels, with PIC values greater than or equal to 0.5, and with major allele differences greater than or equal to 8 bp.

InDels with major allele difference ≥3 bp.

Ontology analysis of genes affected by InDels with PIC ≥ 0.5 and major allele difference ≥3 bp in CDS region.

One set of 41855 InDels with major allele difference ≥ 3 bp and PCR product lengths of 60 100 bp was selected for polyacrylamide gel electrophoresis (Additional file 3: Table S3), while the other set of 14428 InDels with a major allele difference ≥ 8 bp and PCR product lengths of 150 300 bp was selected for agarose gel electrophoresis (Additional file 4: Table S4).

A set of 100 InDel markers (Additional file 6: Table S6) with PIC ≥ 0.5 and a 3 10 bp major allele difference in genomes of two inbred rice cultivars between Nipponbare and 9311 were randomly selected from the developed InDel markers for accuracy and polymorphism validation by the PCR technique.

Finally, we also provided an ontological analysis of the genes affected by I with PIC ≥ 0.5 and a major allele difference ≥3 bp in the CDS region (Additional file 5: Table S5); these characteristics made these InDels valuable for molecular breeding applications.