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Many of the proteins that are initially identified in proteomic studies are major abundant "housekeeping" proteins involved in fundamental metabolic pathways; proteins expressed at low levels, including regulatory proteins, are harder to detect.
Upper panel: coomassie staining illustrates overall uniform protein loading of samples despite great differences in expression of major abundant proteins.
We reported in a previous research about free oligosaccharides such as lactose, sialylhexose, and sialyllactose that we detected as major abundant carbohydrates in control urine samples of four healthy individuals [ 26].
The glycosylation pattern of the major abundant glycans, such as the bG0, bG1 (1,6_bG1 and 1,3_bG1) and bG2 isoforms, was very comparable between GP2013 and the reference product (Fig. 5a).
This inhibition in the synthesis of major OMPs under stress conditions is to maintain an envelope homeostasis, as some of these OMPs constitute major abundant proteins under normal growth conditions.
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This result is in well agreement with previous studies [ 19, 20], and clearly demonstrated that the NURD complex is the major and abundant histone H3N-terminal tail-binding proteins at least in the HeLa nuclear extracts.
Based on the intensities of the protein spots on the 2-DE gels and the MASCOT scores of the proteins identified from GeLC-MS/MS method, the major high abundant proteins were plasma proteins including serum albumin, immunoglobulins, hemoglobins, alpha-1-antitrypsin, and transferrin.
Mass spectrometry analysis of the protein content in the area of the Blue Native gel matching the radioactive complex identified a number of subunits of complex III and of the ATP synthase, i.e. proteins from major and abundant high molecular weight mitochondrial components (Fig. 5B).
Analysis of the next most abundant major repeat class of retrotransposons, LINE/Tad1, revealed that its major peak for sRNAs based on abundance, was at 20 nt followed by 23/24 nt (Additional file 3: Figure S3A).
Lens fiber morphogenesis also requires expression of the highly abundant major intrinsic protein (MIP, or aquaporin-0), which is a water channel and a major lens membrane structural protein (Agre and Kozono, 2003), and the connexins Cx43 (Gja1), Cx46 (Gjand and Cx50 (Gja8), to establish gap junction channels (see Mathias et al., 2010).
The catalytic transformation of cellulose, the major component of abundant and renewable lignocellulosic biomass, into building-block chemicals is a key to establishing sustainable chemical processes.
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