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Of interest, the majority of S. aureus real-time PCR-positive major abscess samples were also PVL real-time PCR-positive (89 %).
Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples.
The main diagnoses were ulcer with acute infection (35% of patients) and major abscess (31%).
None of the 150 major abscess samples were real-time PCR or culture positive for S. dysgalactiae subspecies dysgalactiae.
The presence of PVL was mainly confined to S. aureus isolated from major abscess but not DFI biopsy samples.
One-hundred and fifty major abscess and 128 DFI biopsy samples were collected and microbial DNA was extracted by using the Universal Microbe Detection kit for tissue samples.
Similar(46)
Limit on number of major abscesses to ≤30% of total enrolled.
A mixture of the different ABSSSI disease entities should be included with a cap of 30% on the number of major abscesses enrolled [1].
Significant differences were found between real-time PCR and culture assessment for S. aureus (major abscesses and DFIs), S. pyogenes (major abscesses and DFIs), S. agalactiae (major abscesses), S. dysgalactiae subspecies equisimilis (DFIs), and S. anginosus group (DFIs).
Interestingly, there are differences in the species present in major abscesses and DFIs.
Other identified pathogens were similarly present in both major abscesses and DFIs.
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