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The germination and growth of maize were done with treatments at different concentrations of hematite and ferrihydrite NPs, namely 1, 2, 4, and 6 g/L.
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DNA isolations from maize gluten samples were done with the Promega Wizard Magnetic DNA Purification System for Food according to the manufacturer protocol.
Affymetrix maize genome arrays were done in three biological replicates, using standard Affymetrix protocols (Midi_Euk2V3 protocol on GeneChip Fluidics Station 450; scanning on Affymetrix GSC3000G).
Model predictions for each of the maize and wheat data sets were done in each of 50 random partitions, with 90% of the individuals in the training set and 10% of individuals in the testing set to be predicted.
According to the M&M, most of the infection experiments were done in hybrid maize, while the mutants used are in various (and often mixed) genetic backgrounds.
Extractions were done with 100 mg maize flour.
The identification of maize transcripts was done in the maize transcriptome database available in our laboratory.
Detoxification of aflatoxin contaminated maize grains was done in a pilot plant with aqueous ammonia (1%, v/w).
First work about submergence tolerance of maize seedling was done by Mano et al. [ 105].
For developing drought tolerant maize, selection can be done directly under water stress, indirectly under well-watered (optimal) conditions, or under both optimal and stress conditions [ 6].
The homogenate was clarified by centrifugation at 12.000x g for 5 min at 4°C. Preparation of nuclear and cytoplasmic protein extracts from maize cultured cells was done according to [ 40].
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