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In order to understand the relationships among the 70 genes associated with starch synthesis in Brachypodium, rice, wheat, and maize, we constructed a phylogenetic tree (Additional file 5a).
Using the amino acid sequence alignment of APLs from S. polyrhiza, rice, and maize, we constructed a maximum likelihood phylogeny of the APL family.
Similarly, to validate the newly described zma- MIR169s gene copy in maize, we constructed small RNA libraries from endosperm tissue belonging to cultivars B73, Mo17, and their reciprocal crosses (supplementary table S1, Supplementary Material online).
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We constructed a maize small RNA library using mixed RNAs obtained from ears at four different developmental stages.
Also, we constructed two gRNAs targeting the ZmIPK gene in maize protoplasts, at frequencies of 16.4% and 19.1%, respectively.
We constructed a full-length cDNA library of young maize etiolated seedlings in the transfer vector pAcTMVSVG.
We constructed an ultra-high-dentisy linkage map for the large early generation population in maize.
To investigate the evolutionary history of the GH28 family, we constructed a phylogenetic tree based on protein sequences from Arabidopsis, rice, Brachypodium, sorghum, poplar, and maize.
We constructed bacterial artificial chromosome (BAC) libraries for teosinte (Zea mays ssp. Parviglumis, PI384061) and two maize inbred lines (Yu87-1 and W22).
To investigate the evolutionary relationships and functional associations, we constructed a neighbor-joining tree using the full-length amino acid sequences of CDPKs from grape, Arabidopsis, rice, maize, and poplar (Fig. 4, Additional file 2).
In this study, we constructed a chimeric polyprotein containing Dm-AMP1 with native signal peptide, 16-amino-acid-linker peptide of the Ib-AMP precursor and Rs-AFP2 proteins driven by maize ubiquitin promoter and expressed it in rice.
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