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This study contains several orders of magnitude fewer SNPs than previous maize studies that performed GWAS/joint linkage in the NAM population.
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These reports are also consistent with our findings in the market maize study that show that 88% of market maize was locally grown, and maize bought from local sources had higher aflatoxin levels than did maize bought from sources outside the affected area.
Support for this comes from the results of many Bt maize field studies that have not revealed evidence for direct Cry1Ab-effects on non-Lepidoptera species.
Therefore, it has been used for the quantitative microscopic pollen analyses and assessment of maize pollen deposition in the studies that produced the data reported here [41],[41].
It is known from previous studies that maize and greaves-meal are highly digested in the small intestine [ 8, 39, 40].
The maize studies involved a genetic design that alters the allele frequency in the final nested association mapping population.
CIM-NPCIs were compared to SIs in two recent studies that used maize iRILs to obtain high genetic resolution [20; N. Lauter, W. Zhang, D. Hessel, S. Moose, in preparation].
It can be concluded from those studies that fungal isolates from sorghum could infect maize.
There were no studies that fell into this final category for Bt maize.
Furthermore, we showed in our tri-trophic feeding study that Bt maize expressing Cry1Ab does not cause adverse effects to A. bipunctata larvae under realistic worst case exposure conditions.
During the market maize study, district agricultural officers stated that local consumer demand for market maize in Makueni and Kitui was expected to increase because of depletion of farm household stores and the anticipated failure of the upcoming harvest (Oduor J, personal communication).
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