However, the corresponding maize protein sequence revealed a homology of 64% with the PETF gene of the cyanobacterium Fischerella (data not shown).
Additionally, blast analysis of rice high throughput genomic sequences revealed that maize ZEP1 and ZEP2 genes were closer to each other than to the rice ZEP gene, suggesting a maize specific duplication (data not shown).
Low-stringency BLAST searches of available NCBI nucleotide and protein databases with ID1 and the maize IDD genes (ZmIDD) as query sequences revealed significant similarity to zinc finger proteins from animals and yeast, as well as plants.
The comparison of the Sce_Assembly03 against flcDNA, EST, and genomic sequences revealed a higher homology to barley, Brachypodium, and wheat than to maize, rice, and sorghum which was expected, as rye is phylogenetically more closely related to other members of the Pooideae than to maize, rice, and sorghum [ 54, 55].
Comparisons of genomic DNA sequences revealed two other conserved regions surrounding the Leymus LG3a, rice, and sorghum lax-barrenstalk1 ortholoci, and one of these regions was also present in maize and Leymus LG3b sequences.
The midgut sequences revealed only Tudor domains.
(a) Comparison of deduplicated sequences and total sequences reveals the frequency of repeatedly read sequences.
Sequencing revealed a fairly random distribution of the fragments.
Since a BLAST analysis of the recently sequenced maize genome revealed no tps genes with a high similarity to tps10 and tps23, a potential cross-hybridization of the Northern probes is unlikely.
Subfamily 1 consists of rice, wheat, switchgrass, sorghum, and maize sequences.
Silico3 was predicted from the B73 maize sequences.
