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RNA sequencing demonstrated decreased PRKAR1A expression.
Subfamily 1 consists of rice, wheat, switchgrass, sorghum, and maize sequences.
Silico3 was predicted from the B73 maize sequences.
Maize sequences for design of this chip were predominantly selected from the The Institute for Genomic Research TIGRR) maize gene index [ 33].
The scanned maize sequences represent the whole large sequences available for maize in the public database, i.e., excluding the trace files and the gene-only sequences.
Maize sequences were identified by local BLASTP program as described above.
Transcripts with best matches to viral or maize sequences were removed from further analyses.
The public maize sequence sets fall into three classes: (A) maize whole genome sequences, (B) maize repeats, and (c) maize gene enrichment sequences.
For maize, BAC contigs and annotation data were download from the maize sequence ftp site [ 43].
The PCR primers (see Table 2) were designed based on maize cDNA and genomic sequences from the Maize Sequence Database.
The complete genome sequence of maize was downloaded from the maize sequence database (release version 5b; http://www.maizesequence.org/index.html).html
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