Exact(4)
Unfortunately, we could not yet test whether the purple maize mutants that show anthocyanin formation constitutively have the expected lower lignin content.
The preceding protocol has proven successful for positional cloning a number of maize mutants, and should be effective for a large number of the maize mutants that currently exist.
Three of these mutants have been examined in more detail, and molecular and genetic assays demonstrated that these mutants have both distinct and overlapping phenotypes with previously identified maize mutants that relieve epigenetic transcriptional silencing.
The application of this screen led to the identification of multiple mutants, which exhibit some distinct phenotypes relative to one another and to previously identified maize mutants that can reactivate the silent transgene.
Similar(56)
A recently characterized maize mutant that is specifically deficient in M cell function has provided some insight into how differential gene expression across the two cell types might be achieved.
As part of the ER-stress response, the activation of this turnover mechanism has been observed in genome-wide analyses of Arabidopsis treated with inducers of ER stress and in a transcript-profiling assay of maize endosperm mutants that display a long-term ER stress response [ 34, 35, 45].
Lowered levels of rbcL mRNA in RLSB-silenced Arabidopsis and rlsb-1/rlsb-2 mutantsutantsuggeststhatat RLSB is required for the stabilization of these transcripts.
However, we now include data where we have tested the available maize brown-midrib mutants that are affected in lignin biosynthesis.
In contrast to the multiple associated effects reported here for the rlsb-1/ rlsb-2 mutants, a recent study with a different maize mutant showed that greatly reduced Rubisco accumulation, caused by a defect in the assembly factor RAF1, did not show any effects on other plastid-encoded proteins [ 72].
A class of low lignin mutants that were discovered in maize (first identified in 1924 in Minnesota [ 19]) were the brown midrib mutants (bm).
Many developmental mutants that affect the elaboration of the inflorescence have been isolated in maize [ 21] but, to date, only a few of the corresponding genes have been cloned.
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