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1. Maize mutant to be cloned.
2. Maize inbred lines (see Fig. 2A ), at a minimum B73 and Mo17. 1. Maize mutant to be cloned.
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These approaches and the rapidly decreasing cost of sequencing will likely open many maize mutants to cloning by sequencing in the near future.
With the recent completion of the maize genome sequence (Schnable et al., 2009), positional cloning is rapidly becoming routine, opening up the large and diverse collection of maize mutants to cloning.
Using this approach, an early study mapped a characterized maize mutant glossy3 to the previously known locus at an interval of ~2 Mb.
One of these loci is largely responsible for the plant architecture changes from wild teosinte to cultivated modern maize and is allelic to the recessive maize mutant teosinte branched 1 (tb1).
In the id1 maize mutant the terminal shoot meristem continues to display vegetative (i.e. indeterminate) growth.
Perturbation of cytokinin biosynthesis leads to loss of the SAM (Yanai et al. 2005), whereas mutations in cytokinin response regulators, such as the maize mutant aberrant phyllotaxy1 (abph1) cause larger meristems to form (Jackson and Hake 1999; Giulini et al. 2004).
However, the increased susceptibility of maize bm mutants to U. maydis and the increased leaf areas displaying disease symptoms, provide strong evidence for a role of lignification in disease resistance.
Further studies on the vlo1 maize mutant, including cloning this, gene will help us to understand whether it defines a novel step in the abaxial-adaxial patterning of anthers in maize.
The applicability of BSA to RNA-seq was recently demonstrated by mapping the maize mutant gene gl3[ 39].
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