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To characterize the metabolic fluxes in endosperm, maize kernels were labeled to isotopic steady state using 13C-labeled glucose.
In contrast, in an American study, several volunteer maize kernels were found to be winter-hard in northern latitudes and germinated the following spring [16].
Moreover, several spilled maize kernels were observed around open storage areas of two ports and along truck transportation routes near feed manufacturing plants [21].
The 50 g samples of intact maize kernels were treated in the processor for 30 s, and then were screened over a 7·5 mm round-hole sieve to determine the amount of breakage (fines).
Prior to extrusion, the maize kernels were broken to obtain grits (1−2 mm) which were mixed with lime and water to reach a moisture content of 28 g/100 g.
In a study in Korea [21], imported maize kernels were found to be usually processed and mixed with other components in the animal feed manufacturing plants, and finally consumed in the livestock barns.
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Maize kernels are used as feed stuff for pigs, poultry or cattle fattening.
After loading, the storage areas of maize kernels are cleaned with brushes.
Hence, improving the carotenoid contents in maize kernels is a priority objective for breeders in order to obtain nutritional biofortification outcomes.
The rapid and efficient ultrasound assisted extraction of α-zeins proteins from whole maize kernels was achieved using 70% of ethanol followed by precipitation with acetone.
These findings prove that conventional as well as GM maize kernels are spilled during transportation and handling, and that both have the potential to develop fertile plants.
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