In turn, these gene families contain 213 maize genes in total, 51 of which were also identified in Bolduc et al. (2012).
To identify the TRXM, TAB15, PR1, and bgl genes in maize, total RNA was extracted from the leaves using TRIzol regent (Invitrogen, USA) and RT-PCR was performed using a PrimeScript RT Reagent kit (TaKaRa, Japan) according to the respective manufacturer's instructions.
In maize, a total of 103 patterns leading to 92 products were identified.
In maize, a total of 57,973 AS events, including 22,574 non-redundant AS events, were identified from 5,479 genes (45.5% of multiexonic genes) in all 10 maize libraries, with an average of 4.12 AS events per gene.
To further advance our understanding of the folate metabolism in maize, two representative maize inbred lines with significant differences in total folates in mature seeds were chosen to investigate the expression of folate-related genes and the profiling of folate derivatives during kernel formation.
Then, 1454 common orthologs can be found that are shared by the four species when we use 3884 Ostreococcus tauri BDTs to identify orthologous BDTs in Brachypodium distachyon, Selaginella moellendorfii and maize.> -wrap-foot>> -wrap-foot> In total, 2166 unique BDTs from 1696 maize BDTPs were used to search for orthologous transcripts.
A recent comprehensive study of gene expression in relation to cell wall biosynthesis in maize identified a total of seven CAD gene family members, of which the one examined here was highly expressed in internodes [ 8].
24 out of the 69 genes up-regulated in meiocytes vs. anthers were mitochondrial-encoded, which is a significant proportion considering there are only 58 identified genes in mitochondria in maize [ 29] vs 39,656 genes in total (filtered gene set, version 2).
To this end, two representative maize inbred lines with a significant difference in total folates in dry seeds were chosen.
In hydroponically-grown maize, a total of 47 and 39 QTLs were detected for the traits recorded under limited and adequate supply of Fe, respectively.
