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Comparative transcriptomic and proteomic analyses of maize embryos that had been exposed to camptothecin were conducted.
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In addition, the recent discovery that Viviparous8 (Vp8) encodes a putative peptidase, together with the predominant effect of vp8 mutant on ABA synthesis and turnover in maize embryos, indicate that ABA level is also controlled indirectly through regulation of seed-specific factors [ 27].
Embryos that are not transferred are frozen.
These included unfertilized eggs and embryos that had developmental defects.
President Bill Clinton signed a law restricting research on human embryos that resulted in embryos' destruction.
These results suggest that, in maize embryos, one of the cellular responses to CPT is the arrest of cell division.
Here, we present a detailed comparative analysis of the changes in gene expression that take place in maize embryos after exposure to smoke-water and KAR1.
Based on two RNA-seq data sets (NCBI SRA accession number: SRA060791; SRA035621) generated from 21 DAP (days after pollination) and 25 DAP maize embryos, we obtained a group of BD PROs that can drive reporter gene expression in 20 DAP maize embryos transformed by particle gun bombardment.
Treating maize embryos with GA synthesis inhibitors also decrease both the rate of germination and the fraction of embryos that germinate [ 4].
Interestingly, most of them are involved in the HR repair pathway, suggesting that this is the main mechanism for DSBs repair in maize embryos, at least in response to CPT.
They concluded that sodium azide was differentially metabolized by maize callus and germinating maize embryos using different detoxification and repair mechanisms.
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