Work from several different labs in the last several years has expanded our understanding of the genetic requirements for telomerase-independent telomere maintenance in yeast.
Formation of microtubule architectures, required for cell shape maintenance in yeast, directional cell expansion in plants and cytokinesis in eukaryotes, depends on antiparallel microtubule crosslinking by the conserved MAP65 protein family.
It is therefore likely that an ability to control redox homeostasis is essential for maintenance of yeast metabolisms during fermentation.
YM agar medium (Difco, Detroit, MI, USA) was used for pre-culture and maintenance of yeast strains.
It is noteworthy that PGL has earlier been implicated in optimal mitochondrial functions and maintenance of yeast susceptibility to azole antifungals [36].
FEN1 but not EXO1 is critical in telomeric lagging strand DNA synthesis [6] [8], while both EXO1 and FEN1 contribute to recombination-dependent telomere maintenance in yeast and human cells [9] [14].
The role of PDR18 expression in the maintenance of yeast membrane potential was also analysed.
10.7554/eLife.06807.047 Figure 7. Maintenance of yeast P body integrity requires Hsp104.
ATM is a protein kinase, and its kinase activity is critical for telomere maintenance in yeast (Mallory and Petes 2000).
As Hooper et al. discuss, another gene product, SPG17 or seipin, involved in a different variant of HSP, is implicated in lipid-droplet maintenance in yeast.
Alteration of the mitochondrial adenine nucleotide pool by adenine nucleotide exchange with phosphate was shown to affect different physiological processes, such as glucose metabolism, oxidative phosphorylation, mitochondrial biogenesis and DNA maintenance in yeast or mammals [ 9– 13].
