Given that RS cells express higher level of both Ins1 and Ins2 mRNA (Table 1), the reason for this difference is not clear; one possibility is that chronic secretion induced by maintenance media (which contains 25 mM glucose) resulted in relative insulin depletion that did not completely recover during 3 h incubation in 0 mM glucose (see Materials and Methods for details of insulin ELISA).
It is important to note that some of these genes host more than one miRNA such as minichromosome maintenance complex component 7 (MCM7) which contains miR-106b, 93 and 25.
After infection, cells are maintained in maintenance media which contain 2% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin.
The knockdown and plasmid-control cells were always kept in a maintenance medium which contained 0.5 μg/mL blasticidin.
This may also be due to pre-adaptation of the algae to TAP media (maintenance media of the strains), which contains NH4+ as nitrogen source.
In [5, 6, 7, 8], the outage model is represented by the state transition diagram which contains deterioration, inspection, maintenance and other states.
Investigators also plan to study maintenance records and the locomotive's event recorder, which contains information about its speed, braking and other data.
But, attempts to knock-in genes in some cases have encountered a high background of transformation due to maintenance of unwanted circularization of the transforming DNA, which contains possible autonomously replicating sequence (ARS) activity.
This is compatible with the fact that maintenance of ESCs can be achieved by the addition of exogenous Bmp4 or serum, which contains Bmp4 (Ying et al., 2003).
For example, when fission yeast Schizosaccharomyces pombe, which contains three linear chromosomes, is cultured in the absence of telomere maintenance mechanisms, such as the telomere-capping protein, Pot1, chromosomes can become circularised (Baumann and Cech, 2001).
The first group includes pXO1-like plasmids that share a conserved core region which contains genes that are thought to be involved in plasmid replication and maintenance [40].
