The detailed process of the track maintenance is described as follows.
Often overlooked issues like safety, installation, cleaning and maintenance are described.
Later, current studies using new drugs for HDT (induction, conditioning regimen, consolidation, and maintenance) are described and the promising results of this strategy reported.
Fly stocks, food preparation and maintenance were described previously [ 12, 45].
Details of their maintenance are described in the Supplemental Experimental Procedures.
In most publications the anesthesia maintenance is described as being accomplished with volatile anesthetics or balanced anesthesia [ 10, 11], but Propofol-based anesthesia regimes are also mentioned [ 7, 12].
In these studies partial, i.e. background, integration of few pEPI-vectors was never fully excluded, but the episomal maintenance and replication was described [ 1- 4, 6- 9].
Isolation, characterization and maintenance of rMAPC-1, was described previously [11], [12], [13].
The method used for strain maintenance and inoculum preparation was described by Shafiei et al. [ 4, 11].
In this paper, the systematic development of the maintenance programme for the SLIMMER intervention was described, which might contribute to maintenance of the acquired healthy lifestyle.
