Sentence examples for maintenance under neomycin from inspiring English sources

After reaching confluence, cells from a single well were expanded for maintenance under neomycin selection.

After reaching confluence, cells from a single well were expanded for maintenance under neomycin selection and screened for luciferase activity.

Genotype 1a is a most prevalent genotype of hepatitis C virus in North America yet HCV replication has been studied predominantly with genotype 1b subgenomic replicons under neomycin selection in Huh-7 cells.

A RAW264.7 cell line that stably expresses BirA under neomycin selection has been previously described [ 49].

In relative terms, however, the second PTP induction under Neomycin also caused an ∼twofold PTP.

Transfected cells were placed under neomycin selection, and after two weeks clones emerged.

Trypanosoma brucei procyclic (PF) strain 29 13 with a T7 RNA polymerase under neomycin resistance and tetracycline (TET) repressor under hygromycin resistance was grown in SDM-79 medium with 10% fetal calf serum at 27 °C (Wirtz and Clayton 1995).

The BF single marker strain 427 (Wirtz et al. 1999) with a T7 RNA polymerase and TET repressor under neomycin resistance was grown in HMI-9 medium (Hirumi and Hirumi 1984) under 5% CO2 at 37 °C.

In separate transfection experiments, colonies (obtained from both ARID1B and vector transfectants of MiaPaCa2 and PANC1 cells) were pooled independently following 21 days of selection under Neomycin to perform a colony-formation assay (Supplementary Methods S1).

The cells were cultured in DMEM containing 10% FBS in a 5% CO2 incubator at 37°C under neomycin selection for transfected cells (500 µg/ml), passaged using TrypLE (GIBCO-BRL).

cDNA sequences comprising the open reading frames were cloned into the pLNCX2 retroviral vector (Clontech) to generate overexpressing N2aPK1/2 stable cell lines under neomycin selection as above (Supplementary Material, Table S2).