Sentence examples for maintenance passages from inspiring English sources

Data to be collected: ○ Record LAPC4 xenograft tumor maintenance (passages, tumor incidence, latency, tumor caliper measurements (and calculated volume), tumor weight at harvest, time of harvest, and euthanasia method, calculated density of each tumor based on volume and weight).

The A2780 ovarian cell line was obtained from ECACC (European Collection of Animal Cell Culture), and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2 mM l-glutamine, and 1% (v/v) penicillin (10 k units/mL /streptomycin (10 mg/mL), at 310 K in an humidified atmosphere containing 5% CO2; maintenance passages were done at ca. 80% confluency using trypsin.

Here, we report a chemically defined, scalable culture platform involving co-immobilization of heparin catechol (HepC) and collagen type-1 (Col) for the long-term maintenance (>18 passages) of hESCs in a feeder-free condition.

This may be related to recombination and selection during the maintenance and passage of the stabilates through ticks.

Across the 130 students for whom data were analyzed, results indicated that increased opportunities to practice words led to greater maintenance effects when passages were read seven days later but revealed minimal differences across conditions in students' reading of target words presented within a generalization passage.

After in vitro maintenance for 25 passages, the morphology of the untreated HUC-1 cells was unchanged, but long-term exposure to arsenicals resulted in significant changes.

The design of composite components in the aerospace industry often includes structural discontinuities, such as cutouts, for functional requirements like ventilation, tunnel passage, maintenance and repair.

Reports from other contemporary tuberculosis investigators vary in the particulars of the best procedure to use but also support this principle of virulence maintenance through mouse passaging [27], [28].

For routine hPSC maintenance, hPSCs were passaged with Versene (Life Technologies) every 3 5 days.

To minimize possible phenotypic changes in gCSCs due to prolonged maintenance in vitro and multiple passages, early passage gCSCs were frozen in neurosphere medium containing 5% DMSO and stored at −70°C.

Also, two other pES cell lines generated from oocytes of inbred young C57BL/6 mice displayed telomere maintenance or elongation during passages, like fES cells (N33) (Fig. 1E).