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The results suggest that nucleotide change (mutation) could be kept to a minimum (only one, in this case) if due attention is paid for maintenance of strains, but IS mobilization may be more frequent (two events).
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Middlebrook broth is commonly used in the preparation of inocula for antimicrobial assays, biochemical tests (arylsufatase and tellurite reduction) and for maintenance of stock strains.
The culture medium for enrichment, isolation, and maintenance of strain An4 was prepared as described by Stams et al. (1993), except that (per chlorate was tested without the addition of sulfide.
YM agar medium (Difco, Detroit, MI, USA) was used for pre-culture and maintenance of yeast strains.
This chapter reviews the problems associated with the maintenance of standardized strains of laboratory rodents and highlights the need to control their genetic quality and health status regularly.
The reasons for the maintenance of competing strains in the chemostat remains controversial and is subject to continuing mathematical investigation [41], [42].
Unfortunately, original records describing in detail the maintenance of the strains during the period 1942 1955 were destroyed as per US Army policy at the time (Dr. Mark Wolcott, USAMRIID; personal communication), and the personnel who had first-hand knowledge of the strain passage histories and methods are deceased.
I.I. did the isolation and maintenance of the strains under culture conditions.
Systematic inbreeding and maintenance of inbred strains are therefore key prerequisites for reverse genetic applications in Platynereis.
Yeasts virulence factors contribute for both the maintenance of colonizing strains in addition to damage and cause tissue invasion, thus the establishment of infection occurs.
C. albicans virulence factors contribute for both the maintenance of colonizing strains and tissue invasion, thus the establishment of infection occurs [ 14- 16].
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