Middlebrook broth is commonly used in the preparation of inocula for antimicrobial assays, biochemical tests (arylsufatase and tellurite reduction) and for maintenance of stock strains.
The results suggest that nucleotide change (mutation) could be kept to a minimum (only one, in this case) if due attention is paid for maintenance of strains, but IS mobilization may be more frequent (two events).
However, while low reproductive performance could be ascribed to continuous maintenance of the strain in the laboratory, differences were not observed in 12S and 16S rDNA sequences between the Yeerongpilly strain, the other two Australian strains analyzed in this study (A and B; Tables 4 and 5) and those reported by Murrell et al. [ 35].
Procedures for the isolation of Fsp27 genomic clones, generation of Fsp27-deficient mice, and routine maintenance of mouse strain were essentially the same as previously described [6].
Breeding and maintenance of AB strain zebrafish, as well as collecting and staging of embryos, were done according to standard procedures [27].
Therefore, by intercrossing the MMTV-Luc2 het females and males, we have generated homozygous MMTV-Luc2 animals and used them for further breeding and maintenance of the strain.
Although the reasons for this discrepancy with previous reports remains to be explored, one possibility is that critical mutation(s) other than L100P may have been missed during maintenance of the strain after submission to the RIKEN EAD and/or the single backcrossing of homozygous (L100P/L100P) mutant males obtained from RIKEN to female inbred C57BL/6 J mice.
Maintenance of this strain in the environment, in the neighboring areas of both HG and VZ, could probably be explained by the very important role that bat guano plays in spreading H. capsulatum, as reported by Taylor et al. [ 16], in which the authors mention that H. capsulatum propagules can be dispersed at different distances.
YM agar medium (Difco, Detroit, MI, USA) was used for pre-culture and maintenance of yeast strains.
This chapter reviews the problems associated with the maintenance of standardized strains of laboratory rodents and highlights the need to control their genetic quality and health status regularly.
