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Furthermore, we have demonstrated that ROL-3 is required for seam cell development and the maintenance of seam cell identity.
Together these observations indicate that ROL-3 is not required for establishing seam cell identity per se, but rather is needed for the maintenance of seam cell identity.
Additional studies to characterize the relationship between cell contact and the maintenance of seam cell identity using mutant alleles of rol-3 are likely to be informative.
A hypothesis for these observations is that reestablishing a physical connection after cell division is a requirement for continued maintenance of seam cell identity.
In addition, recent studies have suggested that maintenance of seam cells is important for structural integrity of the vulva Pellis-van Berkell et al, 2005; Smith et al, 2005).
Similar(54)
Alterations in seam cell number could indicate a defect in adoption or maintenance of the seam cell fate, execution of the asymmetric seam cell divisions, or seam cell survival (Gleason and Eisenmann 2010; Gorrepati et al. 2013).
Further, in work reported elsewhere, we showed that the GATA factor encoding genes egl-18 and elt-6 identified by this method are targets of the Wnt pathway in the seam cells and are required for the maintenance of the seam cell "progenitor" fate in one daughter of the larval seam cell asymmetric divisions (Gorrepati et al. 2013).
Furthermore, we characterize a novel requirement for ROL-3 in the development of the seam syncytium, a tissue associated with the process of molting, and demonstrate that ROL-3 is necessary for the maintenance of the seam cell identity.
The present work evaluated the effects of aversive conditioning of SEAM on SEAM, SB, and semen.
Eleven other SEAM measures were each similar before and after aversive conditioning of SEAM.
To understand the nature of seam cell defects in ipla-1 mutants, we first analysed the number of seam cells using scm::gfp.
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maintenance of junction
maintenance of boundary
maintenance of segregation
maintenance of sheet
maintenance of certification
maintenance of power
maintenance of stem
maintenance of user
maintenance of plant
maintenance of crosstalk
maintenance of board
maintenance of population
maintenance of law
maintenance of railway
maintenance of oxygenation
maintenance of biodiversity
maintenance of stability
maintenance of health
maintenance of effort
maintenance of membrane
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