They center on the following, interacting aspects of clinical LC-MS/MS: highly manual workflows, complexity of operation and maintenance of instrumentation, sample throughput limits, insufficient detection sensitivity for some analytes, and problems with detection specificity.
The maintenance of a sample's viability during an 8-month storage at room temperature, together with the finding of higher RAT results in the second extraction, suggests that the kit's reagents remains stable samples with no DNA degradation, as proposed by some works [ 20, 29].
Previous research experience shows that compensation is imperative for the establishment and maintenance of developmental samples.
A longstanding challenge in accurate mechanical characterization of engineered and biological tissues is maintenance of both stable sample hydration and high instrument signal resolution.
Although the farmworker population they are targeting is not as mobile as all farmworkers, they have described the extensive methodologic issues in accessing and maintaining the cohort, including the need for culturally appropriate assessment tools and the use of community workers in recruitment and maintenance of the study sample (Eskenazi et al. 2005).
These results indicated that freeze-thawing cycles appear to be more problematic than long-term storage at −20 or −70°C in terms of sample maintenance.
The advantages and disadvantages of centralized versus decentralized models would need to be considered for such a program taking into account the costs of instruments and their maintenance together with practicalities of sample and/or patient transport and results dissemination.
Given the current estimate for ISS SpaceX Dragon flights at approximately 3-month intervals, this would span three opportunities for sample return after an experiment is terminated and ensure maintenance of RNA and protein sample quality.
These advantages include data collection directly at the action site of the tested drug in different tissues over a long time period, direct sample analysis without extraction steps, maintenance of an equilibrium during sampling and last but not least reduced strain on the tested animal [ 19, 32, 33].
Voucher specimens are unavailable because only a small amount of samples were collected to limit negative impact of sampling on maintenance of the local populations.
