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From a practical perspective, inbreeding simplifies the maintenance of pure lines under laboratory conditions.
The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem (ES) cell cultures.
Since extensive sampling of the entire distribution range was not a feasible option, we took advantage of a large community of specialized and highly-dedicated breeders that have as mission the maintenance of pure genetic strains, the vast majority of these captive strains are endowed with a collection code that identifies the original collection locality [ 4].
However, since seed germination and protocorm development in orchids are dependent on suitable mycorrhizal fungi, and because both species and their hybrids associated with specific sets of mycorrhizal fungi [ 15], the patchy distribution of mycorrhizal fungi that are able to support germination of both pure and hybrid seeds may have further contributed to the maintenance of pure parentals.
The rational for selecting D. tertiolecta as a non-model organism in this study lies in its ability to produce large quantities of lipids and starch (up to 67%and27%7% of organism dry weight, respectively), rapid growth rate in hyper saline environments which aids in maintenance of pure cultures, and lack of a rigid cell wall which eases product extraction and genetic manipulation [ 22- 27].
Similar(55)
RAR participated in the collection, identification and maintenance of the pure bacterial strains.
High Maintenance remains a hit of pure unadulterated joy, its deft characterizations and presentational comedy retaining the same surefootedness it had when it inhabited the purer space of a web series.
(2) However, this is not the case in comparisons between parental species and their interspecific hybrids where the mechanisms underlying gene expression appear to be more conserved during the pupal stage suggesting that the underlying regulatory systems are diverging despite the maintenance of expression levels among pure species.
The successful use of ESC-derived cells for clinical application requires a pure cell population, maintenance of differentiated cell phenotype and constitution of functional tissue in vitro and in vivo [9], [10], [18].
The nanofiber matrix we tested in this study was therefore composed of pure collagen I and showed a satisfying cell proliferation and maintenance of myogenic phenotype on both, orientated and unorientated nanofibers.
The programs can be favorably seen as a form of income maintenance that replaces pure entitlements by requiring parents to commit to behaviors society prefers.
Related(17)
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Justyna Jupowicz-Kozak
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